The Hot air oven sterilization procedure uses dry heat to kill microorganisms on materials such as glassware, powders, oils, and metals. Clean items and place them in the oven. Sterilized at temperatures 170 degrees Celsius for 30 minutes or 160 degrees Celsius for 60 minutes.
Medical devices are sterilized in a variety of ways including using moist heat (steam), dry heat, radiation, ethylene oxide (EtO) gas, vaporized hydrogen peroxide, and other sterilization methods (for example, chlorine dioxide gas, vaporized peracetic acid, and nitrogen dioxide).
Hot Air Oven works on Conduction and Radiation. In the process of conduction, the heat is absorbed by the external item and is passed inwards the next. Application of Hot Air Oven. In the wide list of lab testing instruments, Hot Air Oven plays an important role in sterilization.
Autoclave achieves faster sterilization with superior efficacy against bacterial spores and highly resistant microorganisms due to the combination of pressure and moist heat. Hot Air Oven, while slower, is highly effective against microorganisms in specific items where moisture would be problematic.
Hot air ovens use extremely high temperatures over several hours to destroy microorganisms and bacterial spores. The ovens use conduction to sterilize items by heating the outside surfaces of the item, which then absorbs the heat and moves it towards the center of the item.
Disadvantages of Hot Air Ovens
In comparison to the autoclave or other moist heat sterilization methods, a hot air oven has a lower heat capacity and it's slower in killing microorganisms. It's not suitable to sterilize all equipment, like plastic wares and heat-sensitive materials.
A laboratory hot air oven is widely used in scientific research, pharmaceutical industries, and microbiology labs for sterilization, drying, and heat treatment of glassware in various industries. It operates by circulating heated air to ensure uniform temperature distribution, removing moisture and contaminants.
Incubators generally control temperature between 30°C and 60°C. An oven will be required for temperatures 70°C up to 250°C. If you require temperatures up to 300°C then you will need to have a look at our High Temp Oven range. For temperatures below 30°C, it is recommended you look at our refrigerated incubators range.
The autoclave is still considered the most effective method of sterilization (Figure 11.2. 2). Outside laboratory and clinical settings, large industrial autoclaves called retorts allow for moist-heat sterilization on a large scale.
1) Ensure that the instrument and its surroundings are clean. 2) Load the material to be sterilized or depyrogenated or dried, on the trays provided within the oven, close the door and tighten it with the clamps provided. 3) Connect the plug to the power source and switch on the power. 4) Switch on the mains of oven.
Common sterilization times are 170°C for 30 minutes, 160°C for 60 minutes, or 150°C for 150 minutes. Items are sterilized as heat penetrates from the outside in, destroying cell constituents. Quality controls like Browne's tubes and spore strips ensure proper sterilization.
Hot air ovens are laboratory testing equipment that are used to sterilise materials such as glassware, chemicals, and sealed containers. They are also used for drying, baking, curing, and heat-treating various substances.
Hot air oven sterilizes objects such as glassware (test tubes, flasks, and pipettes), metal instruments (blades, scissors), powders (sulfadiazine, starch, zinc oxide), materials that have oil and glass test tubes.
Hot air ovens use extremely high temperatures over several hours to destroy microorganisms and bacterial spores. The ovens use conduction to sterilize items by heating the outside surfaces of the item, which then absorbs the heat and moves it towards the center of the item.
An autoclave uses pressure and steam to sterilize the contents. It's quick and uses lower temps than air ovens. Hot air ovens use dry heat, high temperatures and longer times to achieve the same result. Both achieve the same result, but do it in very different ways.
Incubators generally control temperature between 30°C and 60°C. An oven will be required for temperatures 70°C up to 250°C. If you require temperatures up to 300°C then you will need to have a look at our High Temp Oven range. For temperatures below 30°C, it is recommended you look at our refrigerated incubator range.
Advantages and disadvantages
They can be more rapid than an autoclave and higher temperatures can be reached compared to other means. As they use dry heat instead of moist heat, some pathogens like prions, may not be killed by them every time, based on the principle of thermal inactivation by oxidation.
Conclusion. In conclusion, hot-air ovens are versatile and widely used in various industries for different applications such as sterilization, drying, aging, baking, and curing. Uniform heating, precise temperature control, cost-effectiveness, and user-friendliness are among the numerous benefits.
Forced convection oven
It allows the sample to absorb heat and dry faster since they are always in contact with hot air.
Medical devices are sterilized in a variety of ways including using moist heat (steam), dry heat, radiation, ethylene oxide (EtO) gas, vaporized hydrogen peroxide, and other sterilization methods (for example, chlorine dioxide gas, vaporized peracetic acid, and nitrogen dioxide).