Hot air ovens use dry heat at 160°C for 2 hours to sterilize materials like glassware and surgical instruments. Autoclaves use high-pressure steam at 121°C for 15 minutes, which is effective for liquids and heat-sensitive materials.
By definition, a hot air oven is a sterilizing machine or device that is used to sterilize the equipment and some other materials using dry heat. It is also known as a forced-air circulating oven. The process of dry heat sterilization using a hot air oven was originally developed by Louis Pasteur.
Hot air ovens use extremely high temperatures over several hours to destroy microorganisms and bacterial spores. The ovens use conduction to sterilize items by heating the outside surfaces of the item, which then absorbs the heat and moves it towards the center of the item.
While autoclaves solely use steam to disinfect, sterilisers can use chemicals, high pressure, filtration, irritation, or a combination of these methods to eliminate living organisms.
An autoclave uses pressure and steam to sterilize the contents. It's quick and uses lower temps than air ovens. Hot air ovens use dry heat, high temperatures and longer times to achieve the same result. Both achieve the same result, but do it in very different ways.
Autoclave: Operates at a lower temperature, usually around 121°C, but uses high pressure (15 psi) to increase the efficiency of steam penetration. This pressure makes autoclaves suitable for sterilizing a wide variety of materials, including liquids and heat-sensitive items.
Hot air oven sterilizes objects such as glassware (test tubes, flasks, and pipettes), metal instruments (blades, scissors), powders (sulfadiazine, starch, zinc oxide), materials that have oil and glass test tubes.
Hot Air Oven are designed to generate heat with blower assisted air re-circulation inside the chamber at temperature from ambient+10°C to 150°C, 200°C or 250°C.
1) Ensure that the fan is in working condition during the operation. 2) Do not spill any chemical or water on the tray of oven. 3) Tong should be use to place/remove the glassware or any material from the oven. 4) Ensure that the Exhaust blower is ON before starting the oven.
Autoclaves operate at high temperature and pressure in order to kill microorganisms and spores. They are used to decontaminate certain biological waste and sterilize media, instruments and lab ware.
A hot air oven uses dry heat between 150-250°C to sterilize items through conduction. Common sterilization times are 170°C for 30 minutes, 160°C for 60 minutes, or 150°C for 150 minutes. Items are sterilized as heat penetrates from the outside in, destroying cell constituents.
A hot air oven is a type of dry heat sterilization. Dry heat sterilization is used on equipment that cannot be wet and on material that will not melt, catch fire, or change form when exposed to high temperatures.
Incubators generally control temperature between 30°C and 60°C. An oven will be required for temperatures 70°C up to 250°C. If you require temperatures up to 300°C then you will need to have a look at our High Temp Oven range. For temperatures below 30°C, it is recommended you look at our refrigerated incubator range.
DISADVANTAGES OF HOT AIR OVEN
1) Approach that takes a long time since heat penetration and germ death happen slowly. 2) Most material cannot be with stand high temperature for example, plastic and rubber products cannot be dry heated Sterilized because the temperatures employed (160°-170°c) are to high for them.
Hot air ovens are laboratory testing equipment that are used to sterilise materials such as glassware, chemicals, and sealed containers. They are also used for drying, baking, curing, and heat-treating various substances.
The two common steam-sterilizing temperatures are 121°C (250°F) and 132°C (270°F).
The disadvantage of autoclaves is that they are unsuitable for heat-sensitive objects and repeated exposure to high humidity and heat may dull sharp, fine cutting instruments, particularly high-grade carbon steel edges of scissors and scalpel blades.
Hot air ovens are electrical devices which use dry heat to sterilize. They were originally developed by Louis Pasteur, and are essentially the same as fan ovens used for cooking food. Generally, they use a thermostat to control the temperature.
In summary, the 15-20 minute autoclave duration is a scientifically validated standard that ensures complete sterilization by accounting for heat penetration, material properties, and microbial resistance. This process is vital for maintaining safety and integrity in laboratory and healthcare settings.
o DO NOT AUTOCLAVE SEALED CONTAINERS OR BAGS.
However, prions, such as those associated with Creutzfeldt–Jakob disease, and some toxins released by certain bacteria, such as Cereulide, may not be destroyed by autoclaving at the typical 134 °C for three minutes or 121 °C for 15 minutes and instead should be immersed in sodium hydroxide (1M NaOH) and heated in a ...
PROHIBITED ITEMS: Autoclaves must not be used with the following: ▪ Corrosives (e.g., acids, bases, phenol, etc.), solvents (e.g., ethanol, methanol, chloroform), or radioactive materials: Contact EHS or review the EHS Program Manual, Section 5.2 - Waste Disposal Procedures for proper decontamination and disposal ...