Cuttings need water for hydration, but also enough oxygen for roots to respire and grow. Using a substrate with adequate air porosity, and using the 1 to 5 moisture scale can help to prevent overwatering that leads to slow rooting and increased risk of disease.
The sooner you can get your cuttings into a more normal environment with air flow and no dome, the better off they'll be. After about a week, remove the dome and monitor your cuttings to see if they begin to wilt. If they do, they're not ready to go dome-less, so try again in 1-2 days.
In general, the rooting medium should be sterile, low in fertility, drain well enough to provide oxygen, and retain enough moisture to prevent water stress.
With the exception of succulents, most cuttings need high humidity in order to grow properly. Until cuttings develop roots, they are very susceptible to drying out. If you don't have a bright area with high humidity, you can create a humid environment around the cutting by placing a clear plastic bag over it.
Warm growing medium temperatures accelerate cell division which leads to faster callusing, root initial development and subsequent root growth. It also speeds up the dry-down rate of the growing medium, which also helps encourage better rooting. The best way to warm the growing medium is through bottom heat.
The reason cuttings rot is that over time, the oxygen level of the water drops unless it's moving or oxygen is added. Not only do plants need oxygen in order to grow roots (and, indeed everything else), but the bacteria that cause rotting thrive in low-oxygen environments.
From stick to root development (Stages 1-3), propagators sometimes use too little water or moisture, but most often provide too much water, which leads to leaching and waterlogged propagation substrates (Fig. 1), uneven rooting and loss of cuttings due to rot (Fig. 2) or botrytis.
Give cuttings bright light but not sun. Keep soil moist but not wet. The cuttings should root within three weeks. Admit air gradually to wean the cuttings from their humid environment, then pot singly before outdoor planting.
Cuttings use energy to form new roots. If the cutting has leaves, most of the energy comes from photosynthesis. Expose these cuttings to bright light, but not direct sunlight, during the rooting period. If you use hardwood cuttings that have no leaves, the energy will come from reserves stored in the woody stem.
Technically, you can transfer your cuttings to soil at any time. In fact, you can actually propagate directly into soil, however, it's much harder to do within your home. When you propagate in soil, you have to keep a good balance of soil moisture, air flow, and humidity.
Several cuttings may be placed together in one container. Be sure to add fresh water as needed until the cuttings are fully rooted. Rooting will generally occur in 3-4 weeks but some plants will take longer. When the roots are 1-2 inches long or longer the cutting is ready to be potted up.
Place your stem cutting in your vessel with enough water to cover the node. The water level will drop due to evaporation, so you should add water every 3-5 days as needed. You can watch the roots develop if you have chosen a clear vessel for your cutting.
Controversially, I'm gonna suggest you don't water them very often. I would start with very evenly moist airy soil – water in sans cutting, and mix it round so it's not saturated at the bottom. Add your cutting, and then mist the surface thoroughly daily (trying not to get the cutting). Twice a day if it's hot or dry.
Carefully remove excess leaves to reduce transpiration, so cuttings do not wilt. However, some foliage should be retained to allow photosynthesis to occur. Keep cuttings moist, cool and shaded (collecting in a damp plastic bag is a good idea) until you are ready to put them in a propagation medium.
Most plants will not root well in full sun, so place the cuttings in a location where they will receive a 50/50 ratio of shade to dappled sunlight. For most plants, cuttings thrive on warmth and humidity, and the growing medium should be kept evenly moist but not drenched while roots develop.
Light provides the energy for callus formation and the subsequent generation of adventitious roots. At the same time, light increases plant temperature and accelerates the drying of leaves, which can quickly dehydrate cuttings. (Under LEDs, this would be less of an issue.)
Try to wait until at least 50% of the cuttings have good rooting before potting them up; however, for some hard-to-root species, you may be lucky with only 10% to 20% success.
Should we allow the cuts to dry out a little before sticking them in medium? No - while herbaceous cuttings are less likely to rot, they also root faster than woody plants because they contain less lignin in their stems. Don't give them time to dry out.
Clip off the leaves on the lower half of the shoot so you have a bare stem to insert into your potting mix. Then, if you want, dip the end of your stem in rooting hormone. This generally helps cuttings root more quickly.
Hydrogen peroxide can help to control fungal growth and fungal infection, such as root rot, by preventing the growth of fungi. It can also help kill fungus and bacteria on plant roots.